Ion Exclusion Chromatography For The Purification of L-Glutamine.

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  • ITOH HISAO
    Technology & Engineering Laboratories, Ajinomoto Co., Inc.
  • NISHI AKIO
    Technology & Engineering Laboratories, Ajinomoto Co., Inc.
  • NARUSE MASAYOSHI
    Technology & Engineering Laboratories, Ajinomoto Co., Inc.

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  • イオン排除クロマトグラフィーによるＬ‐グルタミンの精製法の研究
  • イオン ハイジョ クロマトグラフィー ニヨル L グルタミン ノ セイセイホウ

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Ion exclusion chromatography for the purification of L-glutamine is studied. L-glutamine is usually produced by fermentation and used in pharmaceuticals. By using a model solution of L-glutamine and L-glutamic acid, the optimum cation exchange resin is examined. As a result of the experiments, it is found that a cation exchange resin which has smaller crosslinkage and smaller diameter is better. Ammonium sulfate, L-glutamic acid and pyrrolidonecarboxylic acid, which are usually contained in fermentation broth as impurities, are effectively separated by this method. Moreover, the experimental data of the chromatography is expressed fairly well by the differential equations which express the mass transfer in the fixed bed.

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