Control of Gene Expression from the SUC2 Promoter of Saccharomyces cerevisiae using Two Carbon Sources.

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  • 二種類の炭素源を用いる酵母ＳＵＣ２プロモーターからの遺伝子発現制御
  • 2シュルイ ノ タンソゲン オ モチイル コウボ SUC2 プロモーター カラ

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Abstract

We cultured an oversecretion inutant of Saccharomyces cerevisiae strain which harbors a recombinant plasmid pNA3 containing mouse α-amylase gene fused to SUC2 promoter and signal sequence of 28kDa killer toxin and PGK terminetor. We found that gene expression from the promoter was efficiently derepressed in a medium containing lactic acid as a carbon source. When glucose and lactic acid coexisted in the medium, recombinant yeast grew, using glucose exclusively, and the enzyme was produced using lactic acid after complete depletion of glucose. To control lactic acid concentration at 10 kg/m3, we applied an on-line glucose and lactic acid monitoring and controlling system for jar fermentor culture of this recombinant yeast. By a combination of glucose and lactic acid as carbon sources, effective gene expression from SUC2 promoter could be achieved and 2.23 kat/m3 of a-amylase was produced.

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