書誌事項
- タイトル別名
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- Purification Process for .BETA.-Galactosidase Using Aqueous Two-Phase Partitioning and PEG Fractional Precipitation.
- スイセイ 2ソウ ブンパイホウ ト PEG ブンカク チンデンホウ オ クミア
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説明
Combining aqueous two-phase partitioning and PEG fractional precipitation, a simple and effective purification process for the target intra-cellular enzyme β-galactosidase (β-gal) from E. coli (W 3110) cells was developed based on the evaluated differences between surface properties of β-gal and those of other total soluble proteins. β-gal was partitioned to the PEG rich top phase of the used aqueous two -phase system and finally recovered at high selectivity and yield with the following PEG fractional precipitation mediated by the addition of salt to the separated top phase.
収録刊行物
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- 化学工学論文集
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化学工学論文集 24 (2), 285-290, 1998
公益社団法人 化学工学会
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詳細情報 詳細情報について
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- CRID
- 1390282679487487616
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- NII論文ID
- 10002835389
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- NII書誌ID
- AN00037234
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- COI
- 1:CAS:528:DyaK1cXhvVGhsr8%3D
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- ISSN
- 13499203
- 0386216X
- http://id.crossref.org/issn/0386216X
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- NDL書誌ID
- 4424763
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDLサーチ
- Crossref
- CiNii Articles
- OpenAIRE
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- 抄録ライセンスフラグ
- 使用不可
