Separation of high unsaturated fatty acid by use of supercritical gas chromatography.

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  • 超臨界ガスクロマトグラフィによる高度不飽和脂肪酸の分取
  • チョウ リンカイ ガス クロマトグラフィー ニ ヨル コウド フホウワ シボウ

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The effects of supercritical CO2 density, resin volume and injected sample volume on separations of eicosapentanic acid, obtained from fatty acid methyl esters of fish oil, were studied by use of supercritical CO2 chromatography on an industrial scale. Almost all liquid in resins was extracted, using supercritical CO2. The CO2 volume for separation of eicosapentanic acid depended on supercritical CO2 density and packing resin volume. Product parity depended on supercritical CO2 density and the injected sample volume. Using the supercritical CO2 chromatography method, eicosapentanic acid with a concentration of 90% and a yield of 60% was obtained.

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