Effects of Operational Parameters on Filtration and Recovery of Extracellular Polysaccharides from Culture Broth of <i>Polianthes tuberosa</i> Callus

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  • Mine Koji
    Eco-innovation Research Laboratories, Kao Corporation Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University
  • Ujita Goro
    Kao Chemicals Europe S. L.
  • Taya Masahito
    Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University

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Other Title
  • チューベローズ（<i>Polianthes tuberosa</i>）カルス培養液からの多糖類のろ過分離・回収に及ぼす操作因子の影響
  • チューベローズ(Polianthes tuberosa)カルス培養液からの多糖類のろ過 分離・回収に及ぼす操作因子の影響
  • チューベローズ(Polianthes tuberosa)カルス バイヨウエキ カラ ノ タトウルイ ノ ロカ ブンリ ・ カイシュウ ニ オヨボス ソウサ インシ ノ エイキョウ

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Description

The separation of calli and recovery of extracellular polysaccharides (TPS) by filtration from culture broth of Polianthes tuberosa were studied. The solids (calli or cell debris) to be separated included particles smaller than TPS molecules. Dead-end filtration of calli alone at a constant flow rate produced a compressible cake layer, but the use of filter aids such as diatomite, silica or cellulose powder resulted in an incompressible cake layer under the operational conditions, and the separation of calli and recovery of TPS were achieved. This result is attributable to electrical repulsion among calli, filter aids and TPS molecules. Addition of 3% NaCl and small filter aids (average particle size 27.9 µm) allowed stable calli separation, giving a filtrate of 95% optical transmittance and TPS recovery of almost 100%.

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