Histological Study of Epileptic Focus Induced by Ferrous Chloride

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  • 塩化第一鉄によるてんかん焦点の組織学的研究

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Abstract

From the viewpoint that posttraumatic epilepsy might be caused by iron liberated from hemoglobin we conducted a histopathological study in which ferrous chloride was used as the inducing agent on forty Wistar rats at 8 to 9 weeks of age weighing from 150 to 200 grams.<BR>1) Histological changes in the chronic epileptic foci of the cortex were studied and compared at each stage with changes observed during continuous electroencephalography.<BR>2) Microscopical findings of the focal lesions were consistent with the continuous EEG changes on seizure discharge observed at each stage. Iron stored at the injection sites was beginning to be ingested by the macrophages when the first marked seizure discharge on the EEG developed. At the stage of maximal discharge, the macrophages had invaded deeper and were also detected around the white matter similar to the stage of focus completion.<BR>3) Electron microscopical findings indicated that the nuclei of the nerve cells had become transparent due to chromatolysis and swelling of the protoplasm and the mitochondria was also detected. Many paired cisternae, a formation seen in cells and germ cells undergoing marked mitosis, were detected in the protoplasm of the nerve cells and they increased during the completion stage.<BR>4) Ferrous chloride was detected in the macrophages in the white matter and in the secondary lysosomes of the neurons and the astrocytes. Many secondary lysosomes and fibrous structures were detected in the endfeet of swollen astrocytes, which also tended to increase toward the focus completion stage.<BR>5) It may be presumed that the induced iron, remaining as a residual body, might be related to the causative mechanism in developing an epileptic discharge.

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