鱗翅目昆虫の浮遊性培養細胞の高密度大量培養について
書誌事項
- タイトル別名
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- High density and large scale culture of suspension type of cells from insects.
- 鱗し目昆虫の浮遊性培養細胞の高密度大量培養について
- リンシモク コンチュウ ノ フユウセイ バイヨウ サイボウ ノ コウ ミツド
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説明
Large scale suspension cultures of SES-BoMo-15A, SES-MaBr-1 and SES-MaBr-4 cell lines at high densities in roller bottles or spinner flasks are performed using newly developed media, MM-SFM8 and MM-SFM8P. The maintenance of pH at a constant value was very effective to support higher cell densities. The cell density in conventional spinner flasks increased from 5×105 to 12.1×105cells/ml in 500ml culture medium. When an automatic cell culture apparatus, in which the medium 02 concentration is controlled, fresh medium is continuously supplied and waste is withdrawn to be kept free of cell debris by filtration through a ceramic filter, was used to culture cells in a large scale and at a high density. Thus the cell numbers increased from 1.0×106 to 3.2×106cells/ml in a 500-ml culture.
収録刊行物
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- 日本蚕糸学雑誌
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日本蚕糸学雑誌 65 (1), 7-12, 1996
社団法人 日本蚕糸学会
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詳細情報 詳細情報について
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- CRID
- 1390282679504950272
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- NII論文ID
- 10010964657
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- NII書誌ID
- AN00190300
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- ISSN
- 1884796X
- 00372455
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- NDL書誌ID
- 3937332
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- 本文言語コード
- ja
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- 資料種別
- journal article
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- データソース種別
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- JaLC
- IRDB
- NDLサーチ
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
