昆虫病原細菌<i>Bacillus thuringiensis</i>の extrachromosomal DNAのアガロースゲル電気泳動法による単離とその特性

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タイトル別名
  • Isolation and partial characterization of extrachromosomal DNA from serotypes of Bacillus thuringiensis pathogenic to lepidopteran and dipteran larvae by agarose gel electrophoresis.
  • 昆虫病原細菌Bacillus thuringiensisのextrachromosomal DNAのアガロース電気泳動法による単離とその特性〔英文〕
  • コンチュウ ビョウゲン サイキン Bacillus thuringiensis

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Extrachromosomal DNA molecules of 24 different B. thuringiensis strains were consistently isolated using a procedure which optimizes lysis of the normally lysozyme resistant vegetative cells. The results obtained can be summarized as follows:<br>1. Twenty-four strains of B. thuringiensis contained extrachromosomal DNA molecules of various sizes that were readily visualized with agarose gel electrophoresis and that ranged from >200 to <1 megadaltons (Mdal), depending on the strain.<br>2. A determination of the numbers of extrachromosomal DNA molecules based on agarose gel profiles revealed that there were 1 for sotto and thompsoni, 2 for subtoxicus, entomocidus, ostriniae and darmstadiensis, 3 for dendrolimus and indiana, 5 for galleriae, 6 for canadensis, morrisoni, and israelensis, 7 for wuhanensis, 8 for kenyae, tolworthi, and pakistani, 9 for thuringiensis (BA-068) and dakota, 10 for toumanoffi, 11 for thuringiensis Berliner, 13 for alesti and aizawai (juroi), 14 for kyushuensis, and 16 for kurstaki (HD-1). Such abundance and variety of extrachromosomal DNA elements is a rare phenomenon among bacterial species.<br>3. In serologically identical varieties (thuringiensis Berliner and thuringiensis BA-068, serotype 1; sotto and dendrolimus, serotype 4a, 4b; subtoxicus and entomocidus, serotype 6), only serotype 6 strains show similar extrachromosomal DNA profiles and while a number of strains of B. thuringiensis have some extrachromosomal DNA elements in common, distinct differenecs can be observed in the DNA profiles between serotypes and even among strains of the same serotype.

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