サソリ毒LqhIT2を発現する組み換えバキュロウイルスの殺虫効果

DOI 日本農学文献記事索引 Web Site 参考文献28件
  • 今井 典子
    Laboratory of Molecular Entomology and Baculovirology Laboratory of Applied Entomology, Tokyo University of Agriculture and Technology
  • ALI SOMAIA EL-SAVED
    Department of Entomology, University of California
  • EL-SINGABY NASHAAT RASHED
    Department of Entomology, University of California
  • 岩永 将司
    Laboratory of Molecular Entomology and Baculovirology
  • 松本 正吾
    Laboratory of Molecular Entomology and Baculovirology
  • 岩淵 喜久男
    Laboratory of Applied Entomology, Tokyo University of Agriculture and Technology
  • 前田 進
    Laboratory of Molecular Entomology and Baculovirology Department of Entomology, University of California Core Research for Evolutional Science and Technology (CREST) Project, Japan Science and Technology Corporation Deceaced

書誌事項

タイトル別名
  • Insecticidal effects of a recombinant baculovirus expressing scorpion toxin LqhIT2.
  • Insecticidal effects of a recombinant baculovirus expressing scorpion toxin LphIT2
公開日
2000
資源種別
journal article
DOI
  • 10.11416/kontyushigen1930.69.197
公開者
社団法人 日本蚕糸学会

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説明

A system of the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV) and the silkworm larvae, B. mori, was applied to analyze the potential use of the insect-specific toxin LghIT2 for improving the efficiency of recombinant baculoviruses for pest control. The LghIT2 gene, along with the signal sequence for secretion, was synthesized and transferred into the BmNPV genome to generate the recombinant virus BmLghIT2. Larvae infected with BmLghIT2 showed LghIT2-specific symptoms at 48h post injection (p. i.), stopped feeding at 60h p. i., and became motionless by 84h p. i. Particularly, the larvae infected with BmLghIT2 stopped moving 24h earlier than those infected with BmAaIT, which expressed another insect-specific scorpion toxin AaIT. An N-terminal sequence and mass spectrometric analyses showed that BmLghIT2 produced mature LghIT2. The present results suggest that LghIT2 is more efficient than AaIT for improving the insecticidal activity of baculoviruses.

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