昆虫病原糸状菌Ｂｅａｕｖｅｒｉａ　ｂｒｏｎｇｎｉａｒｔｉｉの蚕およびマウスに対する安全性について

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  • Safety tests for the use of an entomogenous fungus Beauveria brongniartii.
  • コンチュウ ビョウゲン シジョウキン Beauveria brongniart

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In order to utilize as a microbial insecticide the entomopathogenic fungus Beauveria brongniartii (B. tenella) isolated from dead adults of the yellow-spotted longicorn beetle, the pathogenicity to and other harmful effects of this fungus on the silkworm and mouse were investigated in this tests. When 21 isolates of this fungus were inoculated individually to fourth instar larvae of the silkworm by dipping them into a conidial suspension (107-108conidia/ml), all the treated larvae surviued and pupated subsequently. The fungus was not pathogenic to the larvae, while B. bassiana showed a marked pathogenicity. In safety tests for the mouse, the animals were subjected to inraperitoneal injections as well as oral and respiratory administration of a large amount of conidia of this fungus. All the mice survived and exhibited a normal appearance and behavior. Weight gain of the treated mice was almost similar to that of the controls. There was no indication of pathogenicity to and other harmful effects on the mouse. Conidia of the fungus did not germinate at 35°C, and they died within seven days under the thermal condition.

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