屋久島土壌から分離されたB.thuringiensis (2) : 殺虫活性とcry遺伝子

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タイトル別名
  • Study on the isolation and identification of Bacillus thuringiensis in soil samples from Yakushima Island (II) Insecticidal activity and the identification of cry genes.
  • 屋久島土壌から分離されたＢ．ｔｈｕｒｉｎｇｉｅｎｓｉｓ　ＩＩ．　　殺虫活性とｃｒｙ遺伝子
  • ヤクシマ ドジョウ カラ ブンリ サレタ B.thuringiensis 2 サッチュウ カッセイ ト cry イデンシ
  • (II) Insecticidal activity and the identification of cry genes
  • II. 殺虫活性と<i>cry</i>遺伝子

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We previously reported that 53 B. thuringiensis Isolates in soils from Yakushima Island were serologically investigated. In the present study, the Insecticidal activity of these strains against the silkworm Bombyx mori and the mosquito larvae Aedes japonicus was investigated and the identification of cry genes in these strains was conducted using the PCR method.<br>Oligonucleotide primers used for making DNA probes were cry1Aa, cry1Ab, cry1Ac, cry1Ba, cry1Ca, cry1Da, cry1Ea, cry2Aa, cry4Aa, cry1Ba, cryl0Aa and cry 11Aa genes, depending on their specific nucleotide domains.<br>Cry gene profiles of 24 serovar kurstaki strains were the same for crylAa, crylAb and crylAc with a type strain of HD-1, except for cry1Aa the Jano9-2-2 strain composed of cry1Aa and cry1Ab. All of the strains included the cry2Aa gene.<br>In the 14 strains of serovar galleriae, all of the strains included the same crylAb and cry2Aa genes as the type strain.<br>In the other serovar from Yakushima Island, thuringiensis, kenyae and israelensis strains displayed different profiles from the type strains used in this experiment.<br>Serovar kurstaki Jano9-2-2 revealed high insecticidal activity against silkworm larvae compared with the control strain HD-1.<br>Serovar israelensis Aiko2-1-1 also revealed high activity against the Aedes japonicus larvae compared with the type strain. These two strains seem to include novel cry genes.

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