<i>Bacillus thuringiensis</i> subsp. <i>tohokuensis, kumamotoensis</i> ならびに <i>tochigiensin</i> から環状2重鎖DNAの単離

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  • Isolation of covalently closed circular DNA from Bacillus thuringiensis subspp. tohokuensis, kumamotoensis, and tochigiensis.
  • Bacillus thuringiensis subsp.tohokuensis,kumamotoensisならびにtochigiensisから環状2重鎖DNAの単離〔英文〕
  • Bacillus thuringiensis subsp tohokuensi

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Three new serotypes of B. thuringiensis were examined for the presence of covalently closed circular (CCC) DNA molecules using a procedure which optimizes lysis or the normally lysozyme resistant vegetative cells. The results obtained can be snmmarized as follows.<br>Two of the three subspecies of B. thuringiensis contained CCC DNA molecules of several sizes that were readily visualized with agarose gel electrophoresis and that ranged from 120×106 to approximately 2×106 daltons, depending on the strain.<br>A determination of the numbers of plasmid DNAs based on agarose gel profiles revealed there were 3 for subsp. tohokuensis, 4 for subsp. kumamotoensis, and none for subsp tochigiensis.<br>B. thuringiensis subsp. kumamotoensis harbored a giant CCC DNA above molecular weight of 120×106 daltons, however both subsp, tohokunesis and kumamotoensis each harbored 3 relatively small CCC DNAs of similar sizes. The absence of extrachromosomal DNA elements in subsp. tochigiensis in this experiment indicates the contrary with results of many investigators that the production of parasporal body is accompanied by the presence of plasmid DNA. Transformation studies to determine the genetic funtions of plasid DNA are in progress.

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