Increasing curves of two virus DNAs in the midgut epithelium of silkworm infected with Bombyx mori densonucleosis virus type 2 (BmDNV-2).

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  • 家蚕濃核病２型ウイルス（ＢｍＤＮＶ‐２）に感染したカイコの中腸被膜における２種類のウイルスＤＮＡの量的変動
  • カサンノウカクビョウ 2ガタ ウイルス BmDNV-2 ニ カンセン シタ カイコ ノ チュウチョウ ヒマク ニ オケル 2シュルイ ノ ウイルス DNA ノ リョウテキ ヘンドウ

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Abstract

Increasing curves of 6.1 and 6.6kb DNAs of Bombyx mori densonucleosis virus type 2 (BmDNV-2) in the midgut epithelium infected with BmDNV-2 were investigated using a quantitative competitive PCR technique. The 6.1 and 6.6kb DNAs in midgut epithelium increased logarithmically with time, reaching a maximum titer of 500, 000-fold at 48h since the infection (h postinfection, hpi). The maximum titer was maintained until 102hpi after reaching the maximum. Two DNAs during larval ecdysis decreased to 1/625 of maximum titer, indicating that virus-infected cells have dropped out of midgut epithelium. Since increasing curves of two DNAs were very similar, each of two DNAs could be used as template DNA for BmDNV-2 detection by PCR. In insusceptible race of the silkwarm to BmDNV-2 disease, two virus DNAs increased until 12hpi and then decreased rapidly, suggesting that some of viral DNAs have replicated and accumulated in midgut of the insusceptible race. The replication of 6.1kb DNA went ahead of that of 6.6kb DNA in sensitive and insusceptible races of the silkworm. It is suggesting that two DNAs could replicate independently.

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