Changes in SDS-polyacrylamide gel electrophoretic patterns of egg proteins during the embryonic and diapause development of the Japanese oak silkworm, Antheraea yamamai.

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  • FURUSAWA TOSHIHARU
    Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology
  • UEDA TADASHI
    Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology
  • MITSUDA KAZUHIKO
    Ehime Sericultural Experiment Station

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Other Title
  • 天蚕の胚発生および休眠進行に伴う卵蛋白質のSDS-電気泳動パターンの変動
  • 天蚕の胚発生および休眠進行に伴う卵蛋白質のSDS-電気泳動パターンの変動〔英文〕
  • テンサン ノ ハイ ハッセイ オヨビ キュウミン シンコウ ニ トモナウ ラン

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Abstract

In order to define the changes in the protein polypeptides of the Antheraea yamamai egg during embryonic and diapause development, the protein patterns of these eggs were analysed by SDS-polyacrylamide gel electrophoresis. The protein of the eggs, which were obtained immediately after oviposition, were composed mainly of 7 polypeptides (A: 190-200kDa, E: 180kDa, D: 75kDa, E: 68kDa, F: 62kDa, H: 40kDa, J: 33kDa). On day 8 after oviposition, five new bands appeared in the range of 130 to 180kDa, as well as new G (44-46kDa) and I bands (36kDa) were observed with the degradation of bands E, F and H. During the diapause development, two bands between 150 and 160kDa disappeared in the eggs kept at 25°C, but remained in the eggs kept at 5°C. In the course of embryonic development after diapause termination, an abrupt change was observed in the electrophoretic patterns on day 8, after the beginning of post-diapause development. These results suggest that egg proteins may be degraded at certain specific stages during the embryonic development and diapause development.

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