The Relation between Turbidity Spectrum and the Size of Cell and Oil Globule in Suspensions of the Yeast <i>Lipomyces starkeyi</i>

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  • SODEYAMA Masaichi
    Department of Fermentation Technology, Faculty of Engineering, Yamanashi University
  • UZUKA Yasuyuki
    Department of Fermentation Technology, Faculty of Engineering, Yamanashi University
  • TANAKA Kentaro
    Department of Fermentation Technology, Faculty of Engineering, Yamanashi University

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Other Title
  • 酵母<i>Lipomyces starkeyi</i>懸濁液における濁度スペクトル-細胞直径-脂肪球直径の関係
  • コウボ Lipomyces starkeyi ケンダクエキ ニ オケル ダクド スペクトル-サイボウ チョッケイ-シボウキュウ チョッケイ ノ カンケイ ダクドホウ ニ ヨル コウボ Lipomyces starkeyi ノ サイボウ チョッケイ オヨビ サイボウ ナイ シボウキュウ ノ ケントウ 2

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Abstract

Lipomyces starkeyi stores a large amount of lipid as an oil globule within a cell. In this report, the size of the oil globule was measured by means of turbidimetry. Refractive index of the cell was able to be calculated by the Hart-Montroll equation from scattering spectrum which was measured with a special spectrophotometer. This refractive index was correlated to the oil globule size which changed according to the growth. On the other hand, the turbidity spectrum measured with the ordinary spectro-photometer was little related to the oil globule size. So the relation between turbidity <br>ratio (450 mμ/650 mμ) which was calculated from the turbidity spectrum and the cell size nearly agreed with the Hart-Montroll equation when the relative refractive index was 1.04. This shows that turbidity spectrum is useful to determine the size of cell and oil globule. (Received March 13, 1972)

Journal

  • Nippon Nōgeikagaku Kaishi

    Nippon Nōgeikagaku Kaishi 46 (10), 503-508, 1972

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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