澱粉酵素糖化ハイドロールの糖組成について(第2報)

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タイトル別名
  • On the Sugar Composition of Hydrol from the Enzymatic Hydrolyzate of Starch Part II
  • デンプン コウソ トウカ ハイドロール ノ トウ ソセイ ニ ツイテ 2 コウソザイ ニ ヨル ギャクゴウセイ トウルイ ノ ケントウ
  • Reversion Product from D-Glucose by Crude Glucoamylase
  • 酵素剤による逆合成糖類の検討

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Product of reverse action of glucoamylase was prepared by incubating 40% D-glucose solution with 0. 5% of crude glucoamylase at 55°C for 72_??_96 hrs, followed by inactivating the enzyme at 75°C for 10 min. By paper chromatography and paper ionophoresis, nigerose, maltose, isomaltose, laminaribiose, gentiobiose, panose, isopanose and isomaltotriose were detected in the reaction product. Reversion product from 650g of D-glucose was fractionated by a Carbon-Celite column using water and 25_??_15% ethanol as successive elution solvents. Further separation of sugar was carried out by thick paper chromatography and also by Magnesol-Celite column chromatography of the acetylated sugars. In this way, isomaltose, nigerose, maltose, gentiobiose and laminaribiose were identified as their crystalline octaacetates. Panose was identified as crystalline panitol dodecaacetate.<br> These results suggest that the glucobioses and glucotrioses in the hydrol were produced from D-glucose by the reverse action of crude enzyme. The enzyme used in this experiment showed no transglucosylase activity.

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