Toxic Chemical Cross-linker-free Cryosponges Made from Chitosan-Gluconic Acid Conjugate for Chondrocyte Culture
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- Takei Takayuki
- Department of Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University
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- Yoshitomi Hiroki
- Department of Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University
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- Fukumoto Kohei
- Department of Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University
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- Danjo So
- Department of Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University
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- Yoshinaga Takuma
- Division of Clinical Application, Nanpuh Hospital
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- Nishimata Hiroto
- Department of Gastroenterology, Nanpuh Hospital
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- Yoshida Masahiro
- Department of Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University
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抄録
<p>Chitosan has been widely utilized as a porous scaffold material for cartilage regeneration. During the preparation process of chitosan scaffolds, toxic chemical cross-linkers, such as glutaraldehyde, have been used for cross-linking of chitosan molecules. We previously developed chemical cross-linker-free chitosan sponges formed only by freeze-thawing an aqueous solution of chitosan-gluconic acid conjugate (CG). In the present study, we applied the CG cryosponges as scaffolds for cartilage tissue engineering. CG cryosponges with low gluconic acid content showed a defined porous structure and high stability in cell culture medium supplemented with fetal bovine serum that originally contained a low level of lysozyme, a hydrolysis enzyme for chitosan. A favorable mean pore size of the cryosponges for cartilage regeneration (100–300 µm) was accomplished by freezing the CG solution at −30°C. Proliferation and glycosaminoglycan production of bovine chondrocytes were facilitated in plain CG cryosponges compared with those cross-linked with glutaraldehyde. These results demonstrated that the CG cryosponges were promising scaffolds for cartilage tissue engineering.</p>
収録刊行物
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- JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
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JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 50 (2), 142-148, 2017
公益社団法人 化学工学会
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詳細情報
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- CRID
- 1390282679544172800
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- NII論文ID
- 130005241908
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- NII書誌ID
- AA00709658
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- ISSN
- 18811299
- 00219592
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- NDL書誌ID
- 028063724
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- KAKEN
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- 使用不可