Characterization of an <i>in vitro</i> Penetration System for Ocular Iontophoresis

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  • Hikima Tomohiro
    Department of Biosciences and Bioinformatics, Kyushu Institute of Technology
  • Kawakami Masatoshi
    Department of Biosciences and Bioinformatics, Kyushu Institute of Technology
  • Tojo Kakuji
    Department of Biosciences and Bioinformatics, Kyushu Institute of Technology

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  • Characterization of an in vitro Penetration System for Ocular Iontophoresis

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Abstract

<p>We have developed an in vitro penetration cell for transcorneal iontophoretic delivery. The thickness of the diffusion boundary layer δ of this in vitro system was evaluated by benzoic acid dissolution experiments under several mixing conditions of receptor solution. The δ under the condition of magnetic stirrer at 300 rpm in the receptor solution for the present KK cell was 201.0±20.4 µm, which is thinner than that for a Franz diffusion cell (323 µm). Moreover, the mass transfer coefficient km (=D/δ) was calculated by Sh=2+3.45Re0.11Sc0.33; this equation gave the km and δ for the KK cell if the value of the diffusion coefficient D, viscosity μ, and density ρ of receptor solution were known. The penetration rate across cornea from pig in vitro was proportional to the voltage applied to the cornea during iontophoresis. The effect of iontophoresis on the penetration rate was quantitatively investigated after taking into consideration the hydrodynamic diffusion boundary layer.</p>

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