A simple method of chromosome doubling using colchicine in <i>Torenia</i> (Linderniaceae), and the behavior of meiotic chromosomes in amphidiploids

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  • Jiranapapan Jiraporn
    Department of Horticulture, Faculty of Agriculture, Kasetsart University
  • Kikuchi Shinji
    Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University Laboratory of Plant Genetics and Breeding Science, Faculty of Agriculture, Tottori University
  • Manochai Benya
    Department of Horticulture, Faculty of Agriculture, Kasetsart University
  • Taychasinpitak Thunya
    Department of Horticulture, Faculty of Agriculture, Kasetsart University
  • Tanaka Hiroyuki
    Laboratory of Plant Genetics and Breeding Science, Faculty of Agriculture, Tottori University
  • Tsujimoto Hisashi
    Laboratory of Plant Genetics and Breeding Science, Faculty of Agriculture, Tottori University Laboratory of Molecular Breeding, Arid Land Research Center, Tottori University

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  • A simple method of chromosome doubling using colchicine in Torenia (Linderniaceae), and the behavior of meiotic chromosomes in amphidiploids

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Although Torenia fournieri (Linderniaceae) and Torenia baillonii carry 2n=18 and 2n=16 chromosomes, respectively, their F1 hybrid showed eight bivalent chromosomes with chiasmata as a result of interspecific pairing and one univalent chromosome from T. fournieri. To elucidate the behavior of the chromosomes in the amphidiploids, we developed a simple method of chromosome doubling by the application of colchicine combined with the induction of adventitious buds in detached leaves. The frequency of polyploid plants was highest (8%) in the treatment with 15 μmol mol-1 colchicine for 2 days. The meiocytes of the amphidiploids showed extent pairing (i.e., the appearance of bivalent and multivalent chromosomes), including intraspecific and interspecific pairings. This result supports the hypothesis that T. fournieri and T. baillonii have eight homologous chromosomes and that T. fournieri possesses an extra chromosome of unknown origin.

Journal

  • Chromosome Science

    Chromosome Science 14 (1+2), 29-32, 2011

    THE SOCIETY OF CHROMOSOME RESEARCH

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