Validity of a simple vitrification technique for chromosome study of mouse one-cell embryos

DOI 機関リポジトリ Web Site 参考文献20件

書誌事項

公開日
2010
資源種別
journal article
DOI
  • 10.11352/scr.13.45
公開者
一般社団法人 染色体学会

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説明

To evaluate cytogenetic validity of a simple vitrification technique for embryo cryopreservation, mouse 1-cell embryos were vitrified 4, 6, and 8 h after in vitro fertilization (IVF). In addition, chromosomal damage of spermatozoa treated with methyl methanesulfonate (MMS) was estimated using vitrified 1-cell embryos. More than 90% of embryos survived vitrification regardless of the time after IVF. In the 4-h and 6-h groups, some of the surviving embryos swelled after recovery. The incidence of structural chromosome aberrations and aneuploidy in embryos with morphologically normal features did not significantly increase in any group. The vitrification technique preserved 1-cell embryos derived from MMS-treated spermatozoa without alteration of chromosome damage. This technique will enable us to manage the optimal time for chromosome preparation of mouse 1-cell embryos.

収録刊行物

  • Chromosome Science

    Chromosome Science 13 (3+4), 45-48, 2010

    一般社団法人 染色体学会

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