The role of phosphorylation of histone H3 at serine 10 in chromatin condensation in <i>vitro</i>
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- Equilibrina Ilma
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- Krayukhina Elena
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- Inoue Kota
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- Ishikawa Yuya
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- Kawamoto Akihiro
- Graduate School of Frontier Bioscience, Osaka University
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- Suetake Isao
- Institute for Protein Research, Graduate School of Science, Osaka University
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- Takata Hideaki
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- Uchiyama Susumu
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- Fukui Kiichi
- Department of Biotechnology, Graduate School of Engineering, Osaka University
Bibliographic Information
- Other Title
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- The role of phosphorylation of histone H3 at serine 10 in chromatin condensation in vitro
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Description
Post-translational modifications on histone tails play essential roles in modulating chromatin higher order structure. Phosphorylation of histone H3 at serine 10 (H3S10ph) is associated with chromosome condensation. However direct evidence and the molecular mechanism underlying chromosome condensation induced by H3S10ph in human is unclear. Herein, we employed in vitro reconstituted nucleosomal arrays mimicking H3S10ph by substitution S10 to aspartic acid (H3S10D) using human recombinant histones and highly tandem repeats widom 601 sequence. Using analytical ultracentrifugation sedimentation velocity (AUC-SV) analysis and transmission electron microscopy (TEM) observation, we found that there is no significant difference in the local folding and condensation between WT H3 and H3S10D containing nucleosomal arrays. Thus, our results suggest that H3 phosphorylation at serine 10 does not directly play a role in chromatin condensation and thus higher order structure.
Journal
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- Chromosome Science
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Chromosome Science 18 (1-2), 9-14, 2015
THE SOCIETY OF CHROMOSOME RESEARCH
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Details 詳細情報について
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- CRID
- 1390282679545675264
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- NII Article ID
- 130005114061
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- NII Book ID
- AA11163057
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- ISSN
- 21850852
- 13441051
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- NDL BIB ID
- 027010231
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed