Designing Fusion Proteins with Carbohydrate-Binding Modules Having Affinity to Enzymatically Gellable Carboxymethylcellulose Derivative Hydrogel
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- Ashida Tomoaki
- Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University
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- Ojima Yoshihiro
- Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University
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- Sakai Shinji
- Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University
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- Sakka Makiko
- Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University
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- Sakka Kazuo
- Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University
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- Kawakami Koei
- Department of Chemical Engineering, Faculty of Engineering, Kyushu University
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- Taya Masahito
- Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University
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抄録
Carboxymethylcellulose (CMC) hydrogels are promising materials for tissue engineering. In this study, we proposed a method to modify an enzymatically gellable CMC derivative (CMC-Ph) hydrogel by fusing target proteins to a carbohydrate-binding module (CBM). We designed a genetically engineered protein consisting of CBM from Clostridium thermocellum CelJ and green fluorescence protein (GFP) as a model marker. The partition coefficient of CBM-fused GFP, which is the ratio of protein concentration in the hydrogel phase to that in the liquid phase, was about 7 times higher than that of wild-type GFP. In addition, the fusion protein maintained its binding capacity towards the CMC-Ph hydrogel for about two weeks. The high binding capacity of the fusion protein was also preserved in serum-containing medium for animal cell culture. These findings demonstrated that fusing proteins on a CBM is a useful method for creating CMC-derived hydrogels.
収録刊行物
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- JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
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JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 47 (11), 835-840, 2014
公益社団法人 化学工学会
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詳細情報 詳細情報について
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- CRID
- 1390282679545753984
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- NII論文ID
- 130004703870
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- NII書誌ID
- AA00709658
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- ISSN
- 18811299
- 00219592
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- NDL書誌ID
- 026001543
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可