Chromosome analysis in the dioecious cucurbit Coccinia grandis (L.) Voigt

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  • Chromosome analysis in the dioecious cucurbit <i>Coccinia grandis</i> (L.) Voigt

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Karyotype analysis and meiotic behavior in a dioecious cucurbit Coccinia grandis, carrying heteromorphic sex chromosomes, has been carried out using conventional and fluorochrome dyes. Distinct differences in karyotype formula with 15m+4M+2sm+2m: sm+1m: st for male plant and 14m+6M+2sm+2m: st for female plant were observed with orcein staining. In both cases one autosome pair with two constrictions was present. Male plants are seen to possess a heteromorphic Y chromosome with two constrictions which is sufficiently larger than other small sized chromosomes. Giemsa followed by CMA/ DAPI staining through EMA method applied for the first time in this dioecious species, revealed CMA-positive signals in different chromosomes. Detailed meiotic analysis has been carried out from diakinesis and metaphase I to establish genomic stability.

収録刊行物

  • Chromosome Science

    Chromosome Science 15 (1+2), 9-15, 2012

    一般社団法人 染色体学会

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