Karyotype comparison of Indian and Japanese cucumber cultivars by fluorescence <i>in situ</i> hybridization probed with tandem repeat sequences

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  • Zhang Chi
    Laboratory of Genetics, Graduate School of Horticulture, Chiba University
  • Kikuchi Shinji
    Laboratory of Genetics, Graduate School of Horticulture, Chiba University
  • Koba Takato
    Laboratory of Genetics, Graduate School of Horticulture, Chiba University

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  • Karyotype comparison of Indian and Japanese cucumber cultivars by fluorescence in situ hybridization probed with tandem repeat sequences

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Abstract

In the present study, we provide distribution patterns of tandem repetitive DNA sequences on the chromosomes of two cucumber cultivars from India and Japan by FISH method. Four types of tandem repetitive DNA sequence Type I, Type II, Type III and Type IV were adopted as FISH probes. Probe of Type III repeats hybridized to the centromeric regions, while Type I/II and Type IV were detected in the telomeric region in both cultivars. However, different distribution patterns of the repeats were displayed in two cultivars, for example, Type III signals were not detected on the Chromosome 7 of Indian cucumber cultivar and Chromosome 5 of Japanese cucumber cultivar. Also, metaphase chromosomes of Japanese and Indian cucumber cultivars were identified individually by the FISH analysis. These results clarified that these sequences could be used as cytological markers for discrimination of cucumber chromosomes.

Journal

  • Chromosome Science

    Chromosome Science 15 (1+2), 17-21, 2012

    THE SOCIETY OF CHROMOSOME RESEARCH

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