A Single Fluorophore-labeled Aptamer Sensor for the Detection of Interferon Gamma
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- Hashim Siti Norulhuda
- Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
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- Tsuchiya Akira
- Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
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- Kamiya Noriho
- Department of Applied Chemistry, Graduate School of Engineering, Kyushu University Center for Future Chemistry, Kyushu University
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- Sando Shinsuke
- Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
Abstract
Interferon gamma (IFN-γ) is a cytokine that is mainly secreted from immune cells. It plays an important role in regulating neighboring cellular activities through interactions with cell membrane proteins. Because of its biological importance, there has been increasing demand for a biosensor that can respond to IFN-γ. Here, we report a fluorescent aptamer sensor that targets IFN-γ. The 25-nt IFN-γ aptamer was converted into a turn-on fluorescent sensor by labeling the 3′-end with fluorescein. The designed sensor achieved fluorescence sensing of IFN-γ with nanomolar affinity.
Journal
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- Chemistry Letters
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Chemistry Letters 44 (12), 1670-1672, 2015
The Chemical Society of Japan