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A Single Fluorophore-labeled Aptamer Sensor for the Detection of Interferon Gamma

  • Hashim Siti Norulhuda
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
  • Tsuchiya Akira
    Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
  • Kamiya Noriho
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University Center for Future Chemistry, Kyushu University
  • Sando Shinsuke
    Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo

Abstract

Interferon gamma (IFN-γ) is a cytokine that is mainly secreted from immune cells. It plays an important role in regulating neighboring cellular activities through interactions with cell membrane proteins. Because of its biological importance, there has been increasing demand for a biosensor that can respond to IFN-γ. Here, we report a fluorescent aptamer sensor that targets IFN-γ. The 25-nt IFN-γ aptamer was converted into a turn-on fluorescent sensor by labeling the 3′-end with fluorescein. The designed sensor achieved fluorescence sensing of IFN-γ with nanomolar affinity.

Journal

  • Chemistry Letters

    Chemistry Letters 44 (12), 1670-1672, 2015

    The Chemical Society of Japan

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