Influences of Pulsed Electric Fields on the Gene Expression of Pathogenic Bacteria

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  • 病原性細菌の遺伝子発現に対するパルス電界印加の影響に関する研究
  • ビョウゲンセイ サイキン ノ イデンシ ハツゲン ニ タイスル パルス デンカイ インカ ノ エイキョウ ニ カンスル ケンキュウ

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Abstract

Biological and medical field as sterilization with pulsed electric fields or discharges and electroporation have been studied actively in recent years. It is clear that the pulse electric fields bring some sort of influences to organism. But for now, it is not sufficiently clear what influence appears and how mechanism works on the organism by the pulsed electric fields.  The effects of pulsed electric fields on manifestation of RNAs in biological cells were studied with a goal to figure out the mechanism of bioelectrics. The pulsed electric fields were applied to Vibrio parahaemolyticus as pathogenic bacteria and the manifestations of RNAs: TDH, VP1680 and VP1699, which specify pathogenic agents, were analyzed with method of PCR. The manifestation levels of VP1680 increased with the application of pulsed electric fields, and the effect was distinguished when the electric field strength was 14 kV/cm. The electric field strength barely affected the manifestations of TDH and VP1699. It was found that the effects of electric fields were different among RNAs. The manifestation level varied with time when the manifestations are analyzed. These results suggest a probability of selective control on gene expression by pulsed electric fields and new applications such as a development of functional food.

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