High Sensitivity Detection of ATP Using Bioluminescence at An Optical Fiber End

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  • Iinuma Masataka
    Hiroshima University, ADSM, Department of quantum matter MILq : International Project Center for Integration Research on Quantum, Information and Life Science
  • Ushio Yasuaki
    Hiroshima University, Faculty of Science, Department of Physical Science
  • Kuroda Akio
    Hiroshima University, ADSM, Department of molecular biotechnology MILq : International Project Center for Integration Research on Quantum, Information and Life Science
  • Kadoya Yutaka
    Hiroshima University, ADSM, Department of quantum matter MILq : International Project Center for Integration Research on Quantum, Information and Life Science

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  • 光ファイバー先端での生物発光を利用した高感度ＡＴＰ検出
  • ヒカリ ファイバー センタン デノ セイブツ ハッコウ オ リヨウシタ コウカンド ATP ケンシュツ

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Abstract

We investigated the sensitivity of ATP detection based on bioluminescence at an optical fiber end where luciferase molecules were immobilized via silica-binding protein molecules. Luminescence was detected by an avalanche photo diode (APD), with coupling optics to make full use of the merit of compactness, high quantum efficiency and low noise of the APD. The core diameter and the numerical aperture of the optical fiber, as well as the design of the coupling optics, were optimized so as to realize high photon-collection efficiency. A detection limit of about 10-10 M was obtained, which corresponds to 10-15 mol of ATP. A rough estimation shows that the photon count rate is still two orders of magnitude lower than that limited by diffusion or reaction processes, implying a possibility of further improvement of the sensitivity.

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