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Development of Microreactor Array Chip-Based Measurement System for Massively Parallel Analysis of Enzymatic Activity
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- Hosoi Yosuke
- Department of Materials Engineering, School of Engineering The Universituy of Tokyo
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- Akagi Takanori
- Department of Bioengineering, School of Engineering The Universituy of Tokyo Center for NanoBio Integration, The Universituy of Tokyo
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- Ichiki Takanori
- Department of Materials Engineering, School of Engineering The Universituy of Tokyo Department of Bioengineering, School of Engineering The Universituy of Tokyo Center for NanoBio Integration, The Universituy of Tokyo
Bibliographic Information
- Other Title
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- マイクロリアクターアレイチップを用いた酵素機能高効率スクリーニングシステムの開発
- マイクロリアクター アレイ チップ オ モチイタ コウソ キノウ コウコウリツ スクリーニング システム ノ カイハツ
- Development of microreactor array chip‐based measurement system for massively parallel analysis of enzymatic activity
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Description
Microarray chip technology such as DNA chips, peptide chips and protein chips is one of the promising approaches for achieving high-throughput screening (HTS) of biomolecule function since it has great advantages in feasibility of automated information processing due to one-to-one indexing between array position and molecular function as well as massively parallel sample analysis as a benefit of down-sizing and large-scale integration. Mostly, however, the function that can be evaluated by such microarray chips is limited to affinity of target molecules. In this paper, we propose a new HTS system of enzymatic activity based on microreactor array chip technology. A prototype of the automated and massively parallel measurement system for fluorometric assay of enzymatic reactions was developed by the combination of microreactor array chips and a highly-sensitive fluorescence microscope. Design strategy of microreactor array chips and an optical measurement platform for the high-throughput enzyme assay are discussed.
Journal
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- IEEJ Transactions on Electronics, Information and Systems
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IEEJ Transactions on Electronics, Information and Systems 127 (10), 1508-1514, 2007
The Institute of Electrical Engineers of Japan
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Details 詳細情報について
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- CRID
- 1390282679583169664
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- NII Article ID
- 10019958146
- 210000164296
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- NII Book ID
- AN10065950
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- ISSN
- 13488155
- 19429541
- 03854221
- 19429533
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- NDL BIB ID
- 8960164
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- Text Lang
- ja
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- Article Type
- journal article
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- Data Source
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- JaLC
- NDL Search
- Crossref
- CiNii Articles
- KAKEN
- OpenAIRE
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- Abstract License Flag
- Disallowed
