Role of Superoxide Production by Macrophages in the Inflammatory Reaction of the Lung Induced by Asbestos Inhalation.
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- NAKAJIMA Takae
- Div. of Envir. Health Res., Osaka Pref. Inst. of Public Health
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- OHYAMA Masayuki
- Div. of Envir. Health Res., Osaka Pref. Inst. of Public Health
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- INOUE Masayasu
- Dept. of Biochemistry, Osaka City Univ. Med. Sch.
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- HASHIMOTO Masafumi
- Div. of Envir. Health Res., Osaka Pref. Inst. of Public Health
Bibliographic Information
- Other Title
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- アスベスト粒子吸入による気道炎症反応とマクロファージが産生する活性酸素
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Abstract
The present study was performed to clarify the role of active oxygen species produced by macrophages in acute lung injury induced by asbestos (chrysotile) inhalation. Using a sound-wave sifter, we separated chrysotile particles into two types, i.e., fibrous and granular ones, and measured the chemiluminescence (CL) velocity when each type was mixed in a macrophage suspension together with a chemiluminescent agent, lucigenin. The CL was inhibited by superoxide dismutase (SOD), indicating that superoxide is produced in this reaction. The CL intensity was enhanced in the case of fibrous chrysotile but not granular one. These chrysotile particles were instilled into mouse trachea. After 4 days, the lung weight had increased almost twice with the fibrous chrysotile bot not garanular chrysotile. The permeability of the lung capillary, which was examined by a modified Evans blue method, varied in proportion to the lung weight, proving that the increase in lung weight is due to augmented permeability of lung capillaries. Also these inflammatory reactions were suppressed by intravenous SOD injection to the mice. These results suggest that the lung edema induced by asbestos inhalation arises due to particle-geometry-dependent superoxide production by macrophages.
Journal
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- Journal of Japan Society for Atmospheric Environment / Taiki Kankyo Gakkaishi
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Journal of Japan Society for Atmospheric Environment / Taiki Kankyo Gakkaishi 30 (4), 233-242, 1995
Japan Society for Atmospheric Environment
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Details 詳細情報について
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- CRID
- 1390282679593907072
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- NII Article ID
- 130004377110
- 10016363812
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- NII Book ID
- AN10512108
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- ISSN
- 21854335
- 13414178
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed