Internalization of Non-permeant Materials into Cultured Mammalian Cells by Vortex-Stirring in the Presence of a High Molecular Weight Polyacrylic Acid: Direct Evidence of Internalization and Its Dependence on the Cell Lines.
Bibliographic Information
- Other Title
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- Internalization of Non-permeant Materia
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Abstract
Laser scannins confocal fluorescence microscopic analysis provided a direct evidence of the internalization of non-permeant Lucifer Yellow and a dextran of MW 4400 into leukemia L1210 cells when the cells were vortex-stirred in the presence of a high molecular weight polyacrylic acid, A-119 (MW ca. 9×106). The morphology of A-119-treated cells was probed by scanning electron microscopic analysis using 2% glutaraldehyde for fixation. The cells immediately after vortex-stirring with A-119 showed many blebs on the cell surface, indicative of local weakening of the plasma membrane. the blebby surface returned to normal within 5 min at 37°C, but not completely at 0°C even after 20 min. Several cultured cell lines, murine splenocytes, and Ehrlich ascites carcinoma cells were particularly susceptible to permeabilization by this method, although the efficiency varied from one type of cell to another.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 20 (7), 727-731, 1997
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390282679597779712
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- NII Article ID
- 110003639136
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- NII Book ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 4260452
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- PubMed
- 9255409
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed