Development of a Fluorescent Indicator for the Bioimaging of Nitric Oxide.
Bibliographic Information
- Other Title
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- Development of a Fluorescent Indicator
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Abstract
Nitric oxide (NO) has been reported to play various roles as a signal transmitter. However, detailed functions of NO have yet to be clarified. We have developed a fluorescent indicator for NO imaging in living cells. The N-nitrosation of newly designed and synthesized 4-((3-amino-2-naphthyl)aminomethyl)benzoic acid (DAN-1) by NO yielded the highly fluorescent triazole-form. The membrane permeable ester derivative of DAN-1 (DAN-1 EE) was applied to the imaging of NO produced in activated rat aortic smooth muscle cells. After DAN-1 EE has been loaded into cells, the ester bond is hydrolyzed by intracellular esterase, yielding original DAN-1 with less permeability. The fluorescence intensity of the cells loaded with DAN-1 EE increased according to NO production. The imaging method with fluorescent indicators will be significant for the functional clarification of NO in vivo.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 20 (12), 1229-1232, 1997
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390282679599944832
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- NII Article ID
- 110003638986
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- NII Book ID
- AA10885497
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- COI
- 1:CAS:528:DyaK1cXjtFWi
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- ISSN
- 13475215
- 09186158
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- HANDLE
- 10110/00018207
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- NDL BIB ID
- 4371356
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- PubMed
- 9448093
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed