Expression of Low-Molecular-Weight Kininogen in Mouse Vascular Smooth Muscle Cells.
Bibliographic Information
- Other Title
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- Expression of Low-Molecular-Weight Kini
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Abstract
To determine the existence of the kallikrein-kinin system in vascular wall, the expression of low-molecuar-weith (LMW) kininogen, a precursor protein of kinins, was studied in mouse aortic smooth muscle in vivo or in cultured vascular smooth muscle cells (VSMC) derived from mouse aorta in vitro. Although LMW-kininogen mRNA was undetectable in aortic smooth muscle of untreated mice using either Northern blotting or reverse transcription-polymerase chain reactions (RT-PCR) followed by Southern blotting, administration of lipopolysaccharide (LPS; 1 mg/kg, i.v.) induced the expression of LMW-kininogen mRNA at levels detectable by RT-PCR within 12 h. Cultured VSMC not only expressed LMW-kininogen mRNA at levels easily detectable by RT-PCR, but also secreted LMW-kininogen-like protein that was immunoreactive to anti-mouse LMW-kininogen antibody. These results demonstrate that VSMC are a source of LMW-kininogen in the mouse, and suggest the presence of a local kallikrein-kinin system in vascular tissue. LPS-induced up-regulatin of LMW-kininogen expression suggests a role for vascular LMW-kininogen in tissue trauma or inflammation.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 21 (7), 772-774, 1998
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390282679600260480
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- NII Article ID
- 110003639457
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- NII Book ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 4538478
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- PubMed
- 9703266
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed