Thermochemical Studies on the Quantity–Antibacterial Effect Relationship of Four Organic Acids from Radix Isatidis on Escherichia coli Growth

Kong Weijun, Zhao Yanling, Shan Limei, Xiao Xiaohe, Guo Weiying

doi:10.1248/bpb.31.1301

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Thermochemical studies on the quantity-antibacterial effect relationship of four organic acids from Radix Isatidis on Escherichia coli growth
Thermochemical Studies on the Quantity–Antibacterial Effect Relationship of Four Organic Acids from Radix Isatidis on Escherichia coli Growth

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Abstract

In this report, we have investigated the inhibitory action of four organic acids from Radix Isatidis on Escherichia coli growth was investigated at 37 °C by using a microcalorimeter. The four organic acids were: syringic acid, 2-amino-benzoic acid, salicylic acid, benzoic acid. In accordance with thermokinetic model, the pertaining relationships of the drugs, such as growth inhibitory ratio vs. concentration, maximal power-output vs. growth rate constant, growth rate constant vs. concentration, were obtained. Half-inhibitory concentration of the drugs, IC₅₀, was obtained by quantitative analysis. From the view of thermodynamics and molecular structure, the relationship between quantity and effect of the four organic acids has been discussed. The functional groups on phenyl ring had important influence on the antibacterial activities. Our work suggests that microcalorimetry is a fast, simple and more sensitive method that can be easily performed and applied to study the anti-bacterial activities of organic acids from Radix Isatidis on microorganism compared to other biological methods.

Journal

Biological and Pharmaceutical Bulletin

Biological and Pharmaceutical Bulletin 31 (7), 1301-1305, 2008

The Pharmaceutical Society of Japan

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Thermochemical Studies on the Quantity–Antibacterial Effect Relationship of Four Organic Acids from <i>Radix Isatidis</i> on <i>Escherichia coli</i> Growth