Purification of Liver Serine Protease Which Activates Microsomal Glutathione S-Transferase: Possible Involvement of Hepsin
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- Kunii Daisuke
- Laboratory of Functional and Molecular Pharmacology, Graduate School of Medicine, University of the Ryukyus
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- Shimoji Miyuki
- Laboratory of Functional and Molecular Pharmacology, Graduate School of Medicine, University of the Ryukyus
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- Nakama Shinji
- Laboratory of Functional and Molecular Pharmacology, Graduate School of Medicine, University of the Ryukyus
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- Ikebe Masashi
- Laboratory of Functional and Molecular Pharmacology, Graduate School of Medicine, University of the Ryukyus
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- Hachiman Teruyuki
- Laboratory of Functional and Molecular Pharmacology, Graduate School of Medicine, University of the Ryukyus
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- Sato Izumi
- Laboratory of Functional and Molecular Pharmacology, Graduate School of Medicine, University of the Ryukyus
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- Tamaki Atsuko
- Laboratory of Functional and Molecular Pharmacology, Graduate School of Medicine, University of the Ryukyus
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- Yamazaki Kazuko
- Laboratory of Functional and Molecular Pharmacology, Graduate School of Medicine, University of the Ryukyus
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- Aniya Yoko
- Laboratory of Functional and Molecular Pharmacology, Graduate School of Medicine, University of the Ryukyus
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抄録
Rat liver microsomal glutathione S-transferase (MGST1) is known to be activated by trypsin, however, it has not been clarified whether MGST1 is activated by a protease present in liver. In the present study we purified the MGST1 activating protease from liver microsomes and finally identified that the protease is hepsin, a type II transmembrane serine protease. When the protease was incubated with the purified MGST1 or liposomal MGST1 at 4 °C, MGST1 activity was increased 3—4.5 fold after 3—6 d. In electrophoretic and immunoblot analyses after the incubation of MGST1 with the protease MGST1 dimer and its degraded fragment were detected. These results suggest that the rat liver microsomal hepsin functions as MGST1 activating/degrading enzyme.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 29 (5), 868-874, 2006
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679601494016
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- NII論文ID
- 110005602206
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 7892249
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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