Translocation of Lysosomal Cathepsin D Caused by Oxidative Stress or Proteasome Inhibition in Primary Cultured Neurons and Astrocytes

DOI Web Site Web Site 被引用文献1件 参考文献42件
  • Miura Yuri
    Research Team for Functional Genomics, Tokyo Metropolitan Institute of Gerontology
  • Sakurai Yoko
    Research Team for Functional Genomics, Tokyo Metropolitan Institute of Gerontology
  • Hayakawa Masato
    Research Team for Functional Genomics, Tokyo Metropolitan Institute of Gerontology Department of Biological Sciences, Tokyo Metropolitan University
  • Shimada Yukiko
    Research Team for Functional Genomics, Tokyo Metropolitan Institute of Gerontology Research Team for Functional Genomics, Tokyo Metropolitan Institute of Gerontology
  • Zempel Hans
    Research Team for Functional Genomics, Tokyo Metropolitan Institute of Gerontology
  • Sato Yuji
    Research Team for Functional Genomics, Tokyo Metropolitan Institute of Gerontology
  • Hisanaga Shin-ichi
    Department of Biological Sciences, Tokyo Metropolitan University
  • Endo Tamao
    Research Team for Functional Genomics, Tokyo Metropolitan Institute of Gerontology

この論文をさがす

抄録

We reported previously that N-linked glycoproteins were accumulated in the cytosol of the normal aging rat brain, and that one protein had been identified as cathepsin D (Mech. Ageing Dev., 127, 771—778 (2006)). In this study, to elucidate the mechanism of cathepsin D accumulation in the cytosol, we examined the effects of oxidative stress and proteasome inhibition on the apoptosis and subcellular localization of cathepsin D in primary cultured neurons and astrocytes. Using 4′-6-diamidino-2-phenylindole (DAPI)- or Hoechst 33342-staining and annexin V detection, we found that oxidative stress caused by tert-butyl hydroperoxide and proteasome inhibition by lactacystin induced apoptosis in neurons and astrocytes. Furthermore, after cell fractionation, it was demonstrated that cathepsin D was translocated from lysosomes to cytosol under apoptosis-inducing conditions in both cells. These results suggested that oxidative stress and the suppression of proteasome activity triggered the translocation of cathepsin D from lysosomes to cytosol. The possible mechanism of age-related accumulation of cathepsin D in the cytosol of the normal rat brain will be discussed.

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (42)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ