Cloning and Characterization of Sdga Gene Encoding .ALPHA.-Subunit of Heterotrimeric Guanosine 5'-Triphosphate-Binding Protein Complex in Scoparia dulcis
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- Shite Masato
- Graduate School of Medicine and Pharmaceutical Science for Research, University of Toyama
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- Yamamura Yoshimi
- Graduate School of Medicine and Pharmaceutical Science for Research, University of Toyama
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- Hayashi Toshimitsu
- Graduate School of Medicine and Pharmaceutical Science for Research, University of Toyama
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- Kurosaki Fumiya
- Graduate School of Medicine and Pharmaceutical Science for Research, University of Toyama
Bibliographic Information
- Other Title
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- Cloning and characterization of Sdga gene encoding α-subunit of heterotrimeric guanosine 5'-triphosphate-binding protein complex in Scoparia dulcis
- Cloning and characterization of Sdga gene encoding a subunit of heterotrimeric guanosine 5 triphosphate binding protein complex in Scoparia dulcis
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Abstract
A homology-based cloning strategy yielded Sdga, a cDNA clone presumably encoding α-subunit of heterotrimeric guanosine 5′-triphosphate-binding protein complex, from leaf tissues of Scoparia dulcis. Phylogenetic tree analysis of G-protein α-subunits from various biological sources suggested that, unlike in animal cells, classification of Gα-proteins into specific subfamilies could not be applicable to the proteins from higher plants. Restriction digests of genomic DNA of S. dulcis showed a single hybridized signal in Southern blot analysis, suggesting that Sdga is a sole gene encoding Gα-subunit in this plant. The expression level of Sdga appeared to be maintained at almost constant level after exposure of the leaves to methyl jasmonate as analyzed by reverse-transcription polymerase chain reaction. These results suggest that Sdga plays roles in methyl jasmonate-induced responses of S. dulcis without a notable change in the transcriptional level.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 31 (11), 2150-2153, 2008
The Pharmaceutical Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390282679601584512
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- NII Article ID
- 110006968104
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- NII Book ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 9685839
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed