A Glycosidic Spinasterol from Koreana stewartia Promotes Procollagen Production and Inhibits Matrix Metalloproteinase-1 Expression in UVB-Irradiated Human Dermal Fibroblasts
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- Lee Tae Hoon
- Graduate School of Biotechnology and College of Life Science, Kyung Hee University
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- Lee Sang Min
- Graduate School of Biotechnology and College of Life Science, Kyung Hee University
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- Lee Dae-Young
- Graduate School of Biotechnology and College of Life Science, Kyung Hee University
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- Son Youngsook
- Graduate School of Biotechnology and College of Life Science, Kyung Hee University Musculoskeletal Bioorgan Center, Kyung Hee University
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- Chung Dae Kyun
- Graduate School of Biotechnology and College of Life Science, Kyung Hee University Skin Biotechnology Center, Kyung Hee University
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- Baek Nam-In
- Graduate School of Biotechnology and College of Life Science, Kyung Hee University
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- Kim Jiyoung
- Graduate School of Biotechnology and College of Life Science, Kyung Hee University
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Methanol extract of Koreana stewartia leaves (SKE) stimulated collagen production in ultraviolet-B (UVB)-irradiated human fibroblast cells. An active compound was isolated from SKE by successive partitioning and chromatography, and the chemical structure was determined to be 3-O-β-D-glucopyranosylspinasterol (spinasterol-Glc) by spectroscopic characterization. Spinasterol-Glc increased collagen production in the supernatant of UVB-irradiated dermal fibroblast cell cultures in a dose-dependent manner. The effects of spinasteol-Glc on expression of procollagen and matrix metalloproteinase-1 (MMP-1) were further evaluated. We found that the compound stimulated collagen production in UVB-treated fibroblasts than in vehicle-treated control cells by about 3-fold. In addition, we also demonstrate that the compound increased the mRNA and protein levels of procollagen in UVB-treated fibroblast cells, while it inhibited expression of MMP-1. These results indicate that spinasterol-Glc protects fibroblast cells from the adverse effects of UV radiation via stimulation of procollagen synthesis as well as inhibition of MMP-1 expression. Spinasterol-Glc may be useful in the future development of therapeutic and cosmetic applications.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 34 (5), 768-773, 2011
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679601793664
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- NII論文ID
- 130000657794
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 11058055
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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