Electrospray Ionization and Time-of-Flight Mass Spectrometric Method for Simultaneous Determination of Spermidine and Spermine
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- Samejima Keijiro
- Research Institute of Pharmaceutical Sciences, Musashino University
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- Otani Masahiro
- Research Institute of Pharmaceutical Sciences, Musashino University
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- Murakami Yasuko
- Research Institute of Pharmaceutical Sciences, Musashino University
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- Oka Takami
- Research Institute of Pharmaceutical Sciences, Musashino University
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- Kasai Misao
- Research Institute of Pharmaceutical Sciences, Musashino University
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- Tsumoto Hiroki
- Research Institute of Pharmaceutical Sciences, Musashino University
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- Kohda Kohfuku
- Research Institute of Pharmaceutical Sciences, Musashino University
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A sensitive method for the determination of polyamines in mammalian cells was described using electrospray ionization and time-of-flight mass spectrometer. This method was 50-fold more sensitive than the previous method using ionspray ionization and quadrupole mass spectrometer. The method employed the partial purification and derivatization of polyamines, but allowed a measurement of multiple samples which contained picomol amounts of polyamines. Time required for data acquisition of one sample was approximately 2 min. The method was successfully applied for the determination of reduced spermidine and spermine contents in cultured cells under the inhibition of aminopropyltransferases. In addition, a new proper internal standard was proposed for the tracer experiment using 15N-labeled polyamines.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 30 (10), 1943-1946, 2007
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679601854848
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- NII論文ID
- 110006436380
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 8919374
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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