Inhibitory Effect of Glycyrrhizin on the Phosphorylation and DNA-Binding Abilities of High Mobillty Group Proteins 1 and 2 in Vitro
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- SAKAMOTO Ryoko
- Laboratory of Genetical Biochemistry, Graduate School of Medical Sciences, Kitasato University
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- OKANO Maiko
- Laboratory of Genetical Biochemistry, Graduate School of Medical Sciences, Kitasato University
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- TAKENA Hiroko
- Laboratory of Genetical Biochemistry, Graduate School of Medical Sciences, Kitasato University
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- OHTSUKI Kenzo
- Laboratory of Genetical Biochemistry, Graduate School of Medical Sciences, Kitasato University
書誌事項
- タイトル別名
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- Inhibitory Effect of Glycyrrhizin on the Phosphorylation and DNA-Binding Abilities of High Mobility Group Proteins 1 and 2 in Vitro.
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説明
The physiological correlation between glycyrrhizin (GL) and high mobility group proteins 1 and 2 (HMG1/2) and the inhibitory effect of GL on their phosphorylation by three protein kinases (CK-I, CK-II and PKC) were investigated biochemically in vitro. It was found that GL binds directly to HMG1/2, because (i) HMG1/2 have a high affinity with a GL-affinity column; and (ii) GL induces the conformational changes in HMG1/2. Both purified HMG1/2 functioned as phosphate acceptors for these two protein kinases (CK-I and PKC), but not phosphorylated by CK-II. Phosphorylation of HMG1/2 by two protein kinases (CK-I and PKC) was completely inhibited by a glycyrrhetinic acid derivative (oGA) at one-tenth the concentration of GL. Also, the DNA-binding abilities of HMG1/2 were reduced by GL in a dose-dependent manner. These results show that the binding of GL to HMG1/2 results in the inhibition of their physiological activities (DNA-binding ability and phosphorylation by PKC or CK-I) in vitro. The GL-induced inhibition of the physiological activities of HMG1/2 may be involved in the anti-inflammatory effect of GL in vivo.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 24 (8), 906-911, 2001
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679601885440
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- NII論文ID
- 110003638586
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- NII書誌ID
- AA10885497
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- COI
- 1:CAS:528:DC%2BD3MXlvVCmsLs%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 5852166
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- PubMed
- 11510483
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- NDLサーチ
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- PubMed
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- 使用不可