Hydrocortisone Inhibits Cellular Proliferation by Downregulating Hepatocyte Growth Factor Synthesis in Human Osteoblasts
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- Tsunashima Yoshihiko
- Department of Pharmacology, School of Dentistry, Aichi-Gakuin University
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- Kondo Ayami
- Department of Pharmacology, School of Dentistry, Aichi-Gakuin University
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- Matsuda Tomohiro
- Department of Pharmacology, School of Dentistry, Aichi-Gakuin University
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- Togari Akifumi
- Department of Pharmacology, School of Dentistry, Aichi-Gakuin University
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Glucocorticoids have multiple systemic effects that may influence bone metabolism but also directly affect osteoblasts by decreasing their proliferation. Using human osteoblastic SaM-1 cells, we examined whether the effects of hydrocortisone on cellular proliferation are mediated by hepatocyte growth factor (HGF). Human osteoblasts constitutively express both HGF and c-Met, its receptor. Hydrocortisone decreased the gene and protein expression of HGF as well as proliferation in SaM-1 cells. These hydrocortisone (0.01—1 μM)-induced decreases in HGF synthesis and cellular proliferation occurred in a concentration-dependent manner. However, no hydrocortisone (0.01—1 μM)-induced decrease in cellular proliferation was observed in human osteosarcoma-derived cells (HOS and SaOS-2), which are not able to produce HGF. In the cellular proliferation in SaM-1 cells, the decrease was blocked concentration-dependently by exogenously applied HGF (0.01—3 ng/ml). Furthermore, SU11274 (1 μM), a highly specific inhibitor of c-Met, suppressed the proliferation of SaM-1 cells, but not HOS cells. From these results, we concluded that hydrocortisone inhibits the proliferation of SaM-1 cells by interrupting the autocrine/paracrine loop via the downregulation of HGF synthesis.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 34 (5), 700-703, 2011
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679601893504
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- NII論文ID
- 130000657783
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 11057932
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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