Molecular Authentication of Panax ginseng Species by RAPD Analysis and PCR-RFLP.

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  • UM Jae-Young
    College of Pharmacy, Wonkwang University Division of Biological Science, College of Natural Science, Chonbuk National University
  • CHUNG Hwan-Suck
    College of Pharmacy, Wonkwang University
  • KIM Mi-Sun
    College of Pharmacy, Wonkwang University
  • NA Ho-Jeong
    College of Pharmacy, Wonkwang University
  • KWON Hyun-Jeong
    College of Pharmacy, Wonkwang University
  • KIM Jeong-Joong
    Department of Anatomy, Wonkwang University School of Medicine
  • LEE Kang-Min
    Division of Biological Science, College of Natural Science, Chonbuk National University
  • LEE Seung Jae
    The New Life Health Center
  • LIM Jong Phil
    College of Pharmacy, Woosuk University
  • DO Keum-Rok
    College of Oriental Medicine, and Professional Graduate School of Oriental medicine, Wonkwang University
  • HWANG Woo-Jun
    College of Oriental Medicine, and Professional Graduate School of Oriental medicine, Wonkwang University
  • LYU Yeoung-Su
    College of Oriental Medicine, and Professional Graduate School of Oriental medicine, Wonkwang University
  • AN Nyeon-Hyoung
    College of Pharmacy, Wonkwang University
  • KIM Hyung-Min
    College of Pharmacy, Wonkwang University

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In order to develop convenient and reproducible methods for the identification of ginseng drugs at a DNA level, randomly amplified polymorphic DNA (RAPD) and PCR-restriction fragment length polymorphism (PCR-RFLP) analyses were applied within Panax species. To authenticate Panax ginseng among ginseng populations, RAPD analysis was carried out using a 20 mer-random primer. The similarity coefficients among the DNA of ginseng plants analyzed were low, ranging from 0.197 to 0.491. In addition, by using PCR-RFLP analysis, very different fingerprints were obtained within Korean ginseng plants. These results suggest that these methods are able to authenticate the concerned Panax species. Broader application of this approach to authenticate other morphologically similar medicinal materials is rationalized.

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