Molecular Authentication of Panax ginseng Species by RAPD Analysis and PCR-RFLP.
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- UM Jae-Young
- College of Pharmacy, Wonkwang University Division of Biological Science, College of Natural Science, Chonbuk National University
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- CHUNG Hwan-Suck
- College of Pharmacy, Wonkwang University
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- KIM Mi-Sun
- College of Pharmacy, Wonkwang University
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- NA Ho-Jeong
- College of Pharmacy, Wonkwang University
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- KWON Hyun-Jeong
- College of Pharmacy, Wonkwang University
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- KIM Jeong-Joong
- Department of Anatomy, Wonkwang University School of Medicine
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- LEE Kang-Min
- Division of Biological Science, College of Natural Science, Chonbuk National University
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- LEE Seung Jae
- The New Life Health Center
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- LIM Jong Phil
- College of Pharmacy, Woosuk University
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- DO Keum-Rok
- College of Oriental Medicine, and Professional Graduate School of Oriental medicine, Wonkwang University
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- HWANG Woo-Jun
- College of Oriental Medicine, and Professional Graduate School of Oriental medicine, Wonkwang University
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- LYU Yeoung-Su
- College of Oriental Medicine, and Professional Graduate School of Oriental medicine, Wonkwang University
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- AN Nyeon-Hyoung
- College of Pharmacy, Wonkwang University
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- KIM Hyung-Min
- College of Pharmacy, Wonkwang University
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Abstract
In order to develop convenient and reproducible methods for the identification of ginseng drugs at a DNA level, randomly amplified polymorphic DNA (RAPD) and PCR-restriction fragment length polymorphism (PCR-RFLP) analyses were applied within Panax species. To authenticate Panax ginseng among ginseng populations, RAPD analysis was carried out using a 20 mer-random primer. The similarity coefficients among the DNA of ginseng plants analyzed were low, ranging from 0.197 to 0.491. In addition, by using PCR-RFLP analysis, very different fingerprints were obtained within Korean ginseng plants. These results suggest that these methods are able to authenticate the concerned Panax species. Broader application of this approach to authenticate other morphologically similar medicinal materials is rationalized.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 24 (8), 872-875, 2001
The Pharmaceutical Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390282679601896960
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- NII Article ID
- 110003638579
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- NII Book ID
- AA10885497
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- COI
- 1:CAS:528:DC%2BD3MXlvVCms7Y%3D
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 5852083
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- PubMed
- 11510476
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed