Current Application and Technology of Functional Multineuron Calcium Imaging
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- Namiki Shigehiro
- Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo Graduate School of Life and Environmental Sciences, University of Tsukuba
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- Ikegaya Yuji
- Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency
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説明
Here we describe recent applications and technical advancements of functional multineuron calcium imaging (fMCI), which monitors the firing activity of more than a thousand neurons through their somatic Ca2+ signals. fMCI is used for analysis of various neural circuits under normal and pathological conditions. In vitro fMCI is made more sophisticated by using multipoint illumination and scanning technology with spinning-disk and low-laser-intensity imaging, electron-multiplying charge-coupled device cameras, etc. In vivo fMCI is still developing. We review optical technologies for fast scanning imaging, deep tissue imaging, and recording from moving animals.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 32 (1), 1-9, 2009
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679602034944
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- NII論文ID
- 110007021733
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- NII書誌ID
- AA10885497
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- DOI
- 10.1248/bpb.32.1
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 9749248
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可