Current Application and Technology of Functional Multineuron Calcium Imaging

  • Namiki Shigehiro
    Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Ikegaya Yuji
    Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency

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Here we describe recent applications and technical advancements of functional multineuron calcium imaging (fMCI), which monitors the firing activity of more than a thousand neurons through their somatic Ca2+ signals. fMCI is used for analysis of various neural circuits under normal and pathological conditions. In vitro fMCI is made more sophisticated by using multipoint illumination and scanning technology with spinning-disk and low-laser-intensity imaging, electron-multiplying charge-coupled device cameras, etc. In vivo fMCI is still developing. We review optical technologies for fast scanning imaging, deep tissue imaging, and recording from moving animals.

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