HPLC Determination and Pharmacokinetic Study of Homoeriodictyol-7-O-.BETA.-D-glucopyranoside in Rat Plasma and Tissues

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  • Zhao Yunli
    School of Pharmacy, Shenyang Pharmaceutical University
  • Wang Xiaoying
    School of Pharmacy, Shenyang Pharmaceutical University
  • Zhao Yingchun
    School of Pharmacy, Shenyang Pharmaceutical University
  • Gao Xiaoxia
    School of Pharmacy, Shenyang Pharmaceutical University
  • Bi Kaishun
    School of Pharmacy, Shenyang Pharmaceutical University
  • Yu Zhiguo
    School of Pharmacy, Shenyang Pharmaceutical University

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  • HPLC determination and pharmacokinetic study of homoeriodictyol-7-O-β-D-glucopyranoside in rat plasma and tissues
  • HPLC determination and pharmacokinetic study of homoeriodictyol 7 O ベータ D glucopyranoside in rat plasma and tissues

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Abstract

Homoeriodictyol-7-O-β-D-glucopyranoside (HEDT-Glu) was isolated from Viscum coloratum and identified by MS, 1H- and 13C-NMR. A HPLC method was developed for determination of HEDT-Glu in rat plasma and tissues. All biological samples were pretreated by protein precipitation with acetone. Vanillin was selected as internal standard. The mobile phase consisted of methanol–water–glacial acetic acid (45 : 55 : 0.5, v/v/v). Good linearity were observed over the concentration ranges of 0.1—200.0 μg·ml−1 in rat plasma and 0.05—5.0 μg·ml−1 in tissues. Both intra- and inter-day precisions of HEDT-Glu, expressed as the relative standard deviation, were less than 13.1%. Accuracy, expressed as the relative error, ranged from −0.8 to 5.4% in plasma and from −5.6 to 9.4% in tissues. The mean extraction recovery of HEDT-Glu was above 73.17% in biological samples. The described assay method was successfully applied to the pre-clinical pharmacokinetic study of HEDT-Glu. After intravenous administration of HEDT-Glu to rat, AUC and CLtot were 16.04±3.19 μg·h·ml−1 and 0.85±0.17 l·kg−1·h−1, respectively. T1/2,α and t1/2,β were 0.06±0.01 h and 1.27±0.31 h, respectively. HEDT-Glu was cleared from the blood and mainly distributed to the liver and small intestine.

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