Differentiation of Serum-Free Mouse Embryo Cells into an Astrocytic Lineage is Associated with the Asymmetric Production of Early Neural, Neuronal and Glial Markers

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  • Yamaguchi Hideaki
    Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Aomori University
  • Kidachi Yumi
    Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Aomori University
  • Umetsu Hironori
    Laboratory of Food Chemistry, Department of Life Sciences, Junior Collage, Gifu Shotoku Gakuen University
  • Ryoyama Kazuo
    Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Aomori University

書誌事項

公開日
2008
DOI
  • 10.1248/bpb.31.1008
公開者
公益社団法人 日本薬学会

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説明

Serum-free mouse embryo (SFME) cells, the astrocyte progenitor cells in the central nervous system (CNS), were exposed to 10 ng/ml leukemia inhibitory factor (LIF) and 10 ng/ml bone morphogenic protein 2 (BMP2) to induce differentiation, and expression of cell-type specific markers. Nestin, a marker of early neural lineage, βIII-tubulin, a marker of neuronal lineage, oligodendrocyte marker O4 (O4), a marker of oligodendrocytic lineage and glial fibrillary acidic protein (GFAP), a marker of astrocytic lineage, were analyzed. Characteristics of SFME cells, as a CNS progenitor, were identified and a possible mechanism, underlying SFME cell specification into an astrocytic lineage upon differentiation, was investigated. These markers were present, both at the initial proliferative phase and after induction of differentiation. GFAP expression increased strongly upon differentiation, while expression of the other markers changed very little. These results indicate that astrocytic differentiation is associated with the asymmetric production of these markers, rather than through induction of astrocytic markers.

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