Nε-(Carboxymethyl)lysine Proliferated CD34〔+〕 Cells from Rat Choroidal Explant in Culture
-
- Kobayashi Shinjiro
- Department of Pharmacology, Faculty of Pharmaceutical Sciences, Hokuriku University
-
- Shinohara Harumichi
- Department of Anatomy, Kanazawa Medical University
-
- Tsuneki Hiroshi
- Department of Clinical Pharmacology, Toyama Medical and Pharmaceutical University
-
- Nagai Ryoji
- Department of Medical Biochemistry, Kumamoto University Graduate School of Medical Sciences
-
- Horiuchi Seikoh
- Department of Medical Biochemistry, Kumamoto University Graduate School of Medical Sciences
書誌事項
- タイトル別名
-
- N.EPSILON.-(Carboxymethyl)lysine Proliferated CD34+ Cells from Rat Choroidal Explant in Culture
- N イプシロン Carboxymethyl lysine Proliferated CD34 Cells from Rat Choroidal Explant in Culture
この論文をさがす
抄録
Action of Nε-(carboxymethyl)lysine-human serum albumin (CML-HSA) on neovascularization was investigated in cultured rat choroidal explant. Choroidal explants of normal male Wistar rats were cultured in fibrin gel with Dulbecco's modified Eagle medium containing fetal bovine serum in the presence or absence of CML-HSA. Migrated cells were budded from 2nd day in culture and developed from cultured choroidal explants in a time-dependent manner. Budded and developed cells from the choroidal explant had a feature of fibroblasts, which had attenuated long cytoplasmic processes, long ellipsoid nuclei and numerous membrane-bound polymorphic vesicles. Immunostaining of the attenuated cells in fibrin bed with CD34 (a marker protein of vascular endothelial cells and endothelial progenitor cells) failed to disclose positive result. However the cells which were isolated from fibrin bed by collagenase were specifically stained with anti-CD34 antibody. The isolated cells did not form tube-like structures on collagen gel by 3 weeks in culture. CML-HSA significantly increased the number of total isolated cells and CD34+ cells as well as the number of vessel-like structures. These results indicate that CML-HSA overproduced immature blood vessels from cultured choroidal explants in fibrin gel, which consisted of CD34+ cells. The CML-HSA-induced formation of immature blood vessel may be implicated in various choroidal diseases such as age-related macular degeneration.
収録刊行物
-
- Biological & Pharmaceutical Bulletin
-
Biological & Pharmaceutical Bulletin 27 (9), 1382-1387, 2004
公益社団法人 日本薬学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282679602874368
-
- NII論文ID
- 110003608980
-
- NII書誌ID
- AA10885497
-
- ISSN
- 13475215
- 09186158
-
- NDL書誌ID
- 7056586
-
- PubMed
- 15340223
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可