Effect of a Novel Ascorbic Derivative, Disodium Isostearyl 2-O-L-Ascorbyl Phosphate on Human Dermal Fibroblasts: Increased Collagen Synthesis and Inhibition of MMP-1
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- Shibayama Hiroharu
- Department of Dermatology, Jichi Medical University Central Research Center, Toyo Beauty Co., Ltd.
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- Hisama Masayoshi
- Central Research Center, Toyo Beauty Co., Ltd.
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- Matsuda Sanae
- Central Research Center, Toyo Beauty Co., Ltd.
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- Ohtsuki Mamitaro
- Department of Dermatology, Jichi Medical University
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- Iwaki Masahiro
- Department of Pharmacy, School of Pharmacy, Kinki University
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Abstract
The effects of a novel amphiphilic vitamin C derivative, disodium isostearyl 2-O-L-ascorbyl phosphate (disodium 2-(1,3,3-trimethyl-n-butyl)-5,7,7-trimethyl-n-octyl-L-ascorbyl phosphate, VCP-IS-2Na), possessing a C18 alkyl chain attached to a stable sodium L-ascorbic acid 2-phosphate (VCP-Na), on the proliferation of fibroblasts and collagen synthesis, and inhibition of matrix metalloproteinase-1 (MMP-1) in normal human fibroblasts, NHDFs and NB1RGBs, were evaluated. Compared with proliferation of non-treated fibroblasts, VCP-IS-2Na at 50 μM increased proliferation to 123 and 135% of that in NHDFs and NB1RGBs. On the other hand, L-ascorbic acid (vitamin C) and VCP-Na had little effect on proliferation. At a concentration of 5.0—50 μM, VCP-IS-2Na stimulated collagen synthesis with an effectiveness comparable to that of vitamin C and VCP-Na. The amount of type I collagen in the culture medium was increased by treatment with VCP-IS-2Na for 72 h, in a concentration-dependent manner. Maximum increases of 126 and 1067% were seen with VCP-IS-2Na at 50 μM in NHDFs and NB1RGBs, respectively, whereas vitamin C and VCP-Na only had a small effect. VCP-IS-2Na had a small inhibitory effect on MMP-1, but vitamin C did not inhibit MMP-1, and VCP-Na had very little effect. VCP-IS-2Na exerted its collagen synthesis-promoting activity after being converted to vitamin C by phosphatase. This vitamin C promoted proliferation, collagen synthesis and inhibition of MMP-1, which are prolonged through sustained conversion of VCP-IS-2Na.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 31 (4), 563-568, 2008
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390282679603092480
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- NII Article ID
- 110006680485
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- NII Book ID
- AA10885497
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- COI
- 1:CAS:528:DC%2BD1cXmtlaktbc%3D
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 9431522
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- PubMed
- 18379041
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed