Evaluation of Newly Developed Oxygen Meters with Multi-Channels and Disposable Oxygen Electrode Sensors for Antimicrobial Susceptibility Testing

DOI IR HANDLE Web Site Web Site View 1 Remaining Hide 1 Citations 35 References
  • Kitahara Takashi
    Department of Hospital Pharmacy, Nagasaki University School of Medicine
  • Koyama Nao
    Department of Hospital Pharmacy, Nagasaki University School of Medicine
  • Matsuda Junichi
    Department of Laboratory Medicine, Nagasaki University School of Medicine
  • Hirakata Yoichi
    Department of Laboratory Medicine, Nagasaki University School of Medicine
  • Kamihira Shimeru
    Department of Laboratory Medicine, Nagasaki University School of Medicine
  • Kohno Shigeru
    Second Department of Internal Medicine, Nagasaki University School of Medicine
  • Nakashima Mikiro
    Department of Hospital Pharmacy, Nagasaki University School of Medicine
  • Sasaki Hitoshi
    Department of Hospital Pharmacy, Nagasaki University School of Medicine

Search this article

Description

The objective of this study was to investigate the applicability of new oxygen meters with multi-channels and disposable oxygen electrode sensors (DOX-96) on the antimicrobial susceptibility testing of clinical bacterial isolates. The oxygen amount in the wells of 96-well plates was converted into current through electrodes. Bacterial inoculation decreased the oxygen amount in the wells because viable bacteria consume the oxygen. On the other hand, a failure of bacteria to consume oxygen was observed in the presence of potent antimicrobial agents, representing a serious arrest of bacterial metabolism usually leading to stasis or death. Based on these results, the minimum inhibitory concentration was determined by DOX-96 (MICdox). The MICdox showed good agreement with MIC measured by the standard broth microdilution method (98.2%). DOX-96 was also useful for turbid samples such as Mueller-Hinton broth containing 0.1% lipid emulsion. The MICdox in turbid samples showed good agreement with those in clear samples (100.0%). These results indicate that the newly developed DOX-96 is very useful in antimicrobial susceptibility testing even in turbid clinical samples such as colloidal products and turbid biological components.

Journal

Citations (1)*help

See more

References(35)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top