Significant Expression of Functional Human Type 1 Mitochondrial ADP/ATP Carrier in Yeast Mitochondria.
-
- HATANAKA Takashi
- Faculty of Pharmaceutical Sciences, University of Tokushima
-
- TAKEMOTO Yukiyo
- Faculty of Pharmaceutical Sciences, University of Tokushima
-
- HASHIMOTO Mitsuru
- Faculty of Pharmaceutical Sciences, University of Tokushima
-
- MAJIMA Eiji
- Faculty of Pharmaceutical Sciences, University of Tokushima APRO Life Science Institute
-
- SHINOHARA Yasuo
- Faculty of Pharmaceutical Sciences, University of Tokushima
-
- TERADA Hiroshi
- Faculty of Pharmaceutical Sciences, University of Tokushima
Search this article
Abstract
As a first step to characterize the unknown functional properties of the human mitochondrial ADP/ATP carrier (AAC), we tried to express human type 1 AAC (hAAC1) in Saccharomyces cerevisiae. Expression of hAAC1 in yeast mitochondrial membrane was very low, although its transcript level was high. Its expression was improved greatly by replacement of its N-terminal region with the corresponding region of yeast type 2 AAC (yAAC2), as observed with the bovine type 1 AAC (bAAC1). This chimeric hAAC1 showed similar ADP transport activity to that of chimeric bAAC1, corresponding to the transport activity of bAAC1 in bovine heart mitochondria. These results suggested that the N-terminal region of yAAC2 is important for expression of the mammalian carriers in yeast mitochondria. Using the present expression system, studies on the functional properties of the human AAC isoforms in relation to their structures are now possible.
Journal
-
- Biological and Pharmaceutical Bulletin
-
Biological and Pharmaceutical Bulletin 24 (6), 595-599, 2001
The Pharmaceutical Society of Japan
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390282679603177088
-
- NII Article ID
- 110003638522
-
- NII Book ID
- AA10885497
-
- COI
- 1:CAS:528:DC%2BD3MXktFygsbg%3D
-
- ISSN
- 13475215
- 09186158
-
- NDL BIB ID
- 5793233
-
- PubMed
- 11411543
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed