Characterization of Bovine Angiogenin-1 and Lactogenin-Like Protein as Glycyrrhizin-Binding Proteins and Their Vitro Phosphorylation by C-kinase
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- TANIGAWA Kozo
- Laboratory of Clinical Chemistry, School of Allied Health Sciences, Kitasato University
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- FUJIHARA Michio
- Genetical Biochemistry, School of Allied Health Sciences, Kitasato University
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- SAKAMOTO Ryoko
- Genetical Biochemistry, School of Allied Health Sciences, Kitasato University
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- YANAHIRA Shuichi
- Technology and Research Institute, Snow Brand Milk Products Co., Ltd.
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- OHTSUKI Kenzo
- Genetical Biochemistry, School of Allied Health Sciences, Kitasato University
書誌事項
- タイトル別名
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- Characterization of Bovine Angiogenin-1 and Lactogenin-Like Protein as Glycyrrhizin-Binding Proteins and Their in Vitro Phosphorylation by C-kinase.
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説明
Angiogenin-1 (p15, an angiogenesis inducer with RNase activity) and lactogenin-like protein (p17) isolated from partially purified bovine lactoferrin (bLF) preparations were characterized as glycyrrhizin (GL)-binding proteins (gbPs). As expected, bLF-affinity column chromatography confirmed these two gbPs to be bLF-binding proteins. These two purified gbPs exhibited RNase activities when incubated with poly(C) as a substrate. Both GL and glycyrrhetinic acid (GA) at 100 μM significantly inhibited RNase activities of these two gbPs, both of which functioned as phosphate acceptors of C-kinase in vitro. Phosphorylation of p15 and p17 by C-kinase was inhibited by GA in a dose-dependent manner with the 50% inhibition dose (ID50) of approx. 10 μM, whereas GL required a relatively high dose (300 μM) to inhibit significantly it. A GA derivative (oGA, ID50=approx. 0.3 μM) was found to be a potent inhibitor of the C-kinase-mediated phosphorylation of these two gbPs in vitro. In addition, a possible physiological significance of C-kinase on the physiological interaction between bLF and two bLF-binding proteins (p15 and p17) is noted.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 24 (5), 443-447, 2001
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679603206016
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- NII論文ID
- 110003638489
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- NII書誌ID
- AA10885497
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- COI
- 1:CAS:528:DC%2BD3MXjt1alsLc%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 5763767
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- PubMed
- 11379757
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可